Method 1611 describes a quantitative polymerase chain reaction qpcr procedure for the detection of dna from enterococci bacteria in ambient water matrices based on the amplification and detection of. The user should refer to the platform specific instructions for these instruments in the appendices. The assay was performed with the cepheid sunnyvale, calif. Each instrument contains 16 independently programmable modules that can perform four color, realtime fluorometric detection. Acquired cepheid smart cycler using county cri and phep grant funds increase public health capacity for molecular testing ability to accept routine testing as overflow site trialed us epa taqman entercoccus assay pre method a vs. Parameters involved in the conversion of realtime pcr assays. Results and discussion three hundred blood samples were screened for the presence of anti hcv antibodies. In the thresh setting column, enter manual threshold for each channel influenza a. Method 1600 composite sample analysis north beach 4 transects. Evaluation of cepheid smartcycler and euroclone duplicareal time. Brecher, va boston health care system broadcast live from apic c. None of the equipment posted for sale on is owned by medwow, should you have any questions regarding a specific item, please direct them to the appropriate seller by making use of the available communication channels on the items page. Cepheid smartcycler emission spectra of cal fluor and quasar dyes.
Briefly, samples were diluted in star buffer roche. Cepheid has used an icore ceramic heating plate in its smart cycler, heating samples in disposable tubes and. It is the responsibility of employees to ensure that they are using the correct revision of this document by checking the document revision level with that held on the i drive, folder iso. Accurate and statistically verified quantification of. The appropriate data was then pasted into a graphpad prism spreadsheet for linear regression analysis and for determining mean c t values. Ala14, biosan biosan eph electrophoresis in agarose gel. Manual extraction, but automated amplification and detection bd max. Kits chemistries adapted to slow ramp rates can be used in the smart cycler ii martina fricker qpcr 2005 07.
Available realtime rtpcr cycler and kinetic pcr machines. Stock splits are used by cepheid to keep share prices within reasonable numbers to encourage investment. Cepheid smart cycler ii rtpcr model sc2 with 16 well block, including computer with software. To shut down the smartcycler software, choose exit from the file menu, or click the.
Evaluation of the portable cepheid smartcycler realtime. Used cepheid genexpert iv thermal cycler for sale dotmed. During pcr cycling, optical signals from two sequence specific fluorescent probes within the reaction are monitored in real time. L, minicentrifuge with adapter for cepheid reaction tubes, cepheid cooling block, u. The internal quality controls included in each cepheid cartridge check every step of the assay for every patient specimen, not just once a day. Evaluation of cepheid smartcycler and euroclone duplicareal time dual easy ct assay for confirmation of chlamydia. University of michigan property disposition medical equipment. As an early example, following the attack on september 11, 2001, cepheid was awarded. Find out why cepheids solutions are a top choice for fast, accurate, and easy diagnostic testing.
With an average timetoresults of 2040 minutes, the smartcycler system can deliver. Smart cycler ii system, capable of performing cycling parameters described in this sop and simultaneous realtime detection for fam, tet, texas red and cy5 fluorophores, cepheid. The cepheid smartcycler ii is a modular real time pcr system. Roches thermal cycler takes 30 minutes for 40 cycles, and qiagens about 40 minutes for 40 cycles. Pcr diagnostics kits extraction kits qualichem sea. The smartcycler protocol offers a single, closedtube format, which prevents. Evaluation of the portable cepheid smartcycler realtime pcr machine for the rapid diagnosis of footandmouth disease article pdf available in the veterinary record 15020. Pcr, cepheid smart cycler was applied by using qiaamp mini column kit and sacace hcv genotyping kitto estimate the viral load and to investigate the genotypes of hcv. Upload files that contain viruses, corrupted files, or any other similar software or. Smart cycler ii can mimic the conditions of other realtime cyclers. Smart cycler cepheid, dt96 dnatechnology fep fluorescent endpoint detection.
Among them, the simplexa bordetella pertussisparapertussis assay focus diagnostics, the smartcycler bordetella pertussisparapertussis assay cepheid, and bordetella rgene argene. Click show analysis and the wells depicted in the plate layout will turn black. Automated diagnostic results multiple input one output roche lightcycler applied biosystems 7500. Transferring assays between different platforms genequantification. It seems to be working well, but no assays have actually been performed using the. Comparison of smartcycler realtime reverse transcriptionpcr. Cepheid smartcycler ii thermal cycler automated realtime. If the share price of a security gets too high, a company can perform a stock split by issuing all shareholders an extra share, thereby halving the price of an individual share. Objectiveto compare the smartcycler ii system cepheid, sunnyvale, calif results. Consequently, a collaborative project to establish a smartcycler rtpcr for influenza a. The following outlines a rapid and robust method for detecting the human multidrug resistance gene mdr1 snp c3435t using real time polymerase chain reaction pcr. In this process, the probe anneals specifically to the template followed by primer extension and amplification. Upload files that contain software or other material protected by intellectual property laws or by rights of privacy of publicity unless you own or control the rights thereto or have received all necessary consents.
Cepheid smartcycler ii real time instrument with dx software version 1. For assistance, call cepheid technical support at 888 838 3222. Our hours of operation are monday through friday, 8. Subject to the terms and conditions herein, cepheid grants customer a nonexclusive, nontransferable license the license to use one 1 copy of the cepheid smart cycler system software program the software on the computer provided with the smart. In this study the data was analyzed statistically by using spss version 16 wayne and daniel 1995 roger et al 2003. Cepheid smart cycler with quantitect probe rtpcr kit qiagen, valencia, ca. United states department of agriculture agricultural. University of michigan property disposition medical equipment bid june 2017. Problems with fast ramp rate chemistry not compatible. University of michigan property disposition medical equipment bid list. For analysis by the standard curve method of pfaffl 2001, c t data was exported from the cepheid smart cycler as comma separated values files. Calibration required after 1 year or 2,000 runsmodule cepheid ships replacement module to customers onsite intervention required to replace modules.
Cepheid smartcycler ii td pcr manufacturer specifications. Find out why cepheid s solutions are a top choice for fast, accurate, and easy diagnostic testing. Pcr primers used in this study gene primer sequence 18s forward 5. Using quantification cycle values extracted from qpcr instruments, it uses a proven and universally applicable model to give finalized quantification resu. Cepheids genexpert system is the worlds most flexible platform, providing healthcare professionals with a best in class test menu for any setting from the core lab to near patient applications. Wear suitable protective clothing, gloves, and eyeface protection when handling the. The smart leish consists of an assay reagent kit and assay definition files for the polymerase chain reaction pcr instrument platform. No specific limitations were noted in the pi that would affect. Freezer, biomerieux nuclisens easymag system with software version 2. Cphd, california, us was used for the amplification process. Acanthamoeba keratitis ak infection needs to be diagnosed definitively to optimize therapy in order to avoid possible visual impairment. On this page the most prominent realtime pcr cycler are described.
We evaluated the performances of 4 commercial realtime pcr kits for bordetella pertussis is481 sequence detection in nasopharyngeal aspirates by comparison with an inhouse realtime pcr assay. Attantion number of tests includes controls and calibrators for quantitative tests 5 pcr diagnostics kits sexually transmitted infections chlamydia trachomatis packaging arrangement. Manufacturer cepheid 904 caribbean drive sunnyvale, ca 94089 united states. Cepheid smartcycler system software license agreement license grant. One of the pdf files decribes a comparison between lightcycler, smartcycler and abi on sensitivity. Cepheid smart cycler fam, atto 488 cy3, tet, atto 550, tamra texas red, rox, lc 610 cy5, atto 647n, dy649 4plex mx3000p fam, atto 488 tet, atto 520 hex, joe, yakima yellow cy3, atto 550, dy569 tamra, atto 565.
Read the complete quick reference instructions before performing the test. Within the calibrate submenu, select extract background component. Detection of food pathogens using the smart cycler ii. The paper from raja raja et al clinical chemistry 48. Daily use of external controls provides no additional. Rotorgene corbett research, australia, iq icycler iq biorad, usa, mx mx3000 stratagene,usa, sc smart cycler cepheid, usa. A series of studies were preformed evaluating a number of routinely used viral transport medium at a volume of 2 ml. This random access instrument features 16 reaction sites, each of which can be run with a different protocol. Subject to the terms and conditions herein, cepheid grants customer a nonexclusive, nontransferable license the license to use one 1 copy of the cepheid smartcycler system software program the software on the computer provided with the smart. All samples berlin and st etienne were shipped at 20 c to leeds for testing with an inhouse gdh realtime pcr assay.
This pi is located in the microbiology laboratorys pi files. Each county sampled five sites every other week over a nine week period. We have written a visual basic excel macro that facilitates determination and conversion of the appropriate subset of smart cycler table 1. Installation of the smartcycler system is plugandplay, and system expansion only requires an additional usb connection. Fully automated detection of mrsa from nasal swab appropriate collection of nasal swab is very important smart cycler. Pdf evaluation of the portable cepheid smartcycler real. Methods genomic dna was extracted from whole blood. Cepheid s genexpert system is the worlds most flexible platform, providing healthcare professionals with a best in class test menu for any setting from the core lab to near patient applications. The optical system of the unit is centered around the icore, a solid state module which does not require warm up, maintenance, or normalization dye. Fastfinder platform for automated pcranalysis healthtech event eindhoven 24112015.
These samples were collected from the patients who have visited the hospital purposely. Warnings and precautions treat all specimensamples as potentially infectious. Incompatibility of detection format and instrument. The correlation between rtpcr and elisa assay on hepatitis c. Click on the smart cycler icon to start the life science 2d smartcycler software. Cepheid products based on llnl technology include the smart cycler and genexpert instruments which rapidly perform a broad range of genetic tests, from identifying infectious organisms to evaluating atrisk populations for the early detection of disease. Its curvy, raylike forms slice and meld into each other as they spin and rock in a seemingly random motion. Plate from the file menu, designate the plate type as background, and click ok. Leinster acknowledged that he brought out circumstances owners guide pdf relating to windoms demeanor at the time of the. Office of water 4303t 1200 pennsylvania avenue, nw. Users should thoroughly read the method in its entirety before preparation of reagents and commencement of the method to identify differences in protocols for different platforms.
The university of michigan has the listed equipment for sale on a bid basis. Cepheid enabling access to molecular diagnostic testing. Store the xpert xpress sarscov2 cartridges at 228 c. Cepheid xpert mrsa assay package insert pi contains system performance data and describes testing principle and procedure, qc recommendations, and limitations. Wet lab smartcycler wetlab2 research on stationresearch on. Prevalence and associated risk factors of hepatitis c. Pdf the ability of the portable cepheid smartcycler realtime pcr machine to detect footandmouth disease fmd virus sensitively and. Researchers can receive results within 24 hours of. Maintenance of genexpert fondazione centro san raffaele.
Computer is windows 2000 with cepheid smart cycler version 2. Fast with the smartcycler system, you can optimize pcr denaturation, annealing and extension temperatures and times in a single run. The thin especiallyformed reaction vessels made of polypropylene are pushed into a small slot to establish contact with the ce. By automating highly complex and timeconsuming manual procedures, the companys solutions deliver a better way for institutions of any size to perform sophisticated genetic testing for organisms and geneticbased. The primary outcome of interest was the presence and frequency of ms2 andor fluorescent. The ability of the portable cepheid smartcycler realtime pcr machine to detect footandmouth disease fmd virus sensitively and accurately was evaluated by comparing the results of the analyses of nasal swab and serum samples from experimentally infected animals with those obtained from the realtime pcr assay currently in use in the laboratory. Jun 06, 2018 optics data was exported from the cepheid smart cycler as comma separated values files.
The cycle threshold for all positive results was recorded. Comparison of smartcycler realtime reverse transcriptionpcr assay in a. The kit contains sufficient reagents, in lyophilized bead form, to qualitatively assay 50 clinical samples for both leishmania genus and l. It is imperative that all users read the genexpert. We have written a visual basic excel macro that facilitates determination and conversion of the appropriate subset of smart cycler optics data to a logarithmic format for subsequent analysis. Manufacturer specifications smartcycler ii td, cepheid please note.
Customer contacts cepheid to order an xpert calibration kit before the end of the calibration period. The cepheid smart cycler ii system is an integrated dnarna amplification and detection instrument based on the microprocessorcontrolled. Cepheid is a wooden kinetic sculpture named after pulsating stars. Parameters involved in the conversion of realtime pcr assays from the abi prism 7700 to the cepheid smartcycler ii. Cepheid is a leading molecular diagnostics company dedicated to improving healthcare by developing, manufacturing, and marketing accurate yet easytouse molecular systems and tests. Evaluation of four commercial realtime pcr assays for. For the qualitative detection and identification of respiratory syncytial virus and human metapneumovirus viral rna extracted from nasal swab and nasopharyngeal swab specimens. Pdf evaluation of the portable cepheid smartcycler realtime. In 2010, a total of 10 stations that were sampled in 2009 were revisited.
The smartcycler dx system is a rapid, realtime thermal cycler used for identifying dna or rna from prepared biological samples. View and download genexpert xpert mtb rif user manual online. The smarttube optical windows are set at 90 degree angles for the ideal optical read. A positive control with ms2 rna and a negative control of pcr water was included in each run. The smarttube optical windows are set at 90 degree angles for the ideal optical read path. Sas programs for realtime rtpcr having multiple independent. Evaluation of the smartcycler ii system for realtime detection of. Cepheid smartcycler system software license agreement. Every icore module con tains a highly thermal conductive ceramic plate, a fan and two small optical blocks. Workquality assurance project plan njdep new jersey. However, the thermal mass and conductivity of the air still cause some delay 10. As cepheid is set into motion by its spring and magnetic escapement mechanism, it creates a unique visual display of dancing shapes and mesmerizing patterns.
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